Polyclonal Antibodies for the Specialized Signaling G protein, Gbeta5

Researchers at NIDDK have developed polyclonal antibodies against the G protein, Gbeta5. Gbeta5 is a unique and highly specialized G protein that exhibits much less homology than other Gbeta isoforms (~50%) and is preferentially expressed in brain and neuroendocrine tissue. It is expressed prominently in the neuronal cell membrane, as well as in the cytosol and nucleus. Although this distribution pattern suggests that Gbeta5 may shuttle information between classical G protein-signaling elements at the plasma membrane and the cell interior, its function in the brain is largely unknown.

The antibodies were separately generated in rabbits to KLH-conjugates of peptides from the N-terminus of Gbeta5 (antibody ATDG) and the C-terminus of Gbeta5 (antibody SGS). The antibodies can be used for immunoblotting (ATDG, SGS), and immunoprecipitation (ATDG). They can be used to facilitate our understanding of the unique biology and function of Gbeta5 in brain and neurons.