Technology ID
TAB-2957

Chicken Polyclonal Antiserum to the Nitrone Spin Trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO)

E-Numbers
E-108-2013-0
Lead Inventor
Mason, Ronald (NIEHS)
Co-Inventors
Ehrenshaft, Marilyn (Estate of) (NIEHS)
Applications
Research Materials
Lead IC
NIEHS
ICs
NIEHS
Biological radicals resulted from oxidative stress has been implicated in human diseases, such as cancer and aging. There is, however, a paucity of reliable methods for in vivo or ex vivo detection of either radical formation, the end-products of radical formation or susceptibility for radical formation. The chicken polyclonal anti-DMPO can be used to detect the stable nitrone end-product of protein and DNA radicals in ELISA assays, blot analyses and confocal microscopy. This chicken polyclonal anti-DMPO antiserum will supplement both the mouse monoclonal anti-DMPO and the rabbit polyclonal anti-DMPO. The availability of anti-DMPO from three different sources will greatly expand the ability of researchers to use anti-DMPO in conjunction with other antibodies.
Commercial Applications
  • The chicken polyclonal anti-DMPO can be used to detect the stable nitrone end-product of protein and DNA radicals in ELISA assays, blot analyses and confocal microscopy.
Competitive Advantages
  • DMPO is one of the most frequently used spin traps to detect free radicals and has been cited in over 1000 articles (PubMed). The availability of anti-DMPO of different origins (rabbit, mouse, chicken) will increase the ability of the researcher to use anti-DMPO in conjuction with other antibodies of interest.
Licensing Contact:
Choudhry, Vidita
vidita.choudhry@nih.gov